![]() ![]() Standard HPLC is the most widely used liquid chromatography type across various industries. We found it three years later and put it back on it pumped bubbles for a couple minutes and from that point on it was perfectly fine. High-performance liquid chromatography methods are developed on stationary phase particles between 3-5 µm and run at operating pressures up to 600 bar with flow rates between 1-2 mL/min. We actually lost a column under a GC for three years. I don’t believe that is true, in fact I have data to say that that is not true. I know that people say that if a column dries out that the column is ruined. But once you hydrate that column again, the column is going to be fine. You will see bubbles coming out for a little while. Once you pressurize the column the gasses will dissolve so they will disappear. ![]() At that point you can switch to liquid and start pumping liquid through. So, open the purge valve to remove all of the air bubbles. I would start with 100% acetonitrile or 100% methanol and pump all the air out of the lines. But that is a long term issue.īut in the short term simply worry about getting liquid back into your instrument. Now, I don’t want you to run your instrument for long periods of time when it is dry, because if the HPLC pump runs dry your piston will rub against the pump seal, and that will eventually wear out. NONSENSE! Your column is fine the instrument is fine. The new Refractive Index Detector RI 2012 series provide autopurge and autozero capabilities, as well as RS232 and USB communication to acquire data directly. People are out there saying, “Air in your column! Your column is destroyed! You just threw away a $500 column.” Here’s my first statement: Don’t freak out! Don’t worry about it, it is not as nearly as bad as you think. ![]()
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